Extraction of 11-nor-9-carboxy-tetrahydrocannabinol from hydrolyzed urine by ISOLUTE SLE+ prior to GC-MS analysis

This application note describes the extraction of 11-nor-9-carboxy-THC from a urine matrix, prior to GC/MS analysis. Carboxy-THC is the primary metabolite of THC, a key indicator of illicit marijuana usage. In urine, ~80% of the carboxy-THC metabolite is present in the form of its glucuronide metabolite. Therefore, to effectively quantify the THC-COOH, urine is hydrolyzed before extraction. This application note describes optimized extraction of urine samples prepared by either enzymatic or base hydrolysis.