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Biotage Supported Liquid Extraction (SLE) consumables

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Overview


Sample preparation using Supported Liquid Extraction

The Supported Liquid Extraction (SLE) process is analogous to traditional liquid-liquid extraction (LLE) and utilizes the same water immiscible solvent systems for analyte extraction. However, instead of shaking the two immiscible phases together, the aqueous phase is immobilized on an inert diatomaceous earth based support material and the water immiscible organic phase flows through the support, alleviating many of the liquid handling issues associated with traditional LLE such as emulsion formation. As a result recoveries are often higher and demonstrate better reproducibility from sample to sample.

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Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.

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Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns
ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional Liquid Liquid Extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion formation and reduces sample preparation time by half.

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Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis.

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Converting a liquid-liquid extraction method for vitamin D to a Supported Liquid Extraction plate format
The objective of this study was to determine feasibility of transferring a validated Liquid-Liquid Extraction (LLE) method to a Supported Liquid Extraction (SLE) method using Biotage ISOLUTE SLE+ 96-well plates (400 uL sample capacity). The laboratory supporting this study processes ~550 samples per month supporting vitamin D analysis in plasma and serum (mixed samples / not separated). During this evaluation, it was determined that sample processing time was reduced by ~50% for a de-identified patient set (n=30, split and processed by both methods). Towards high throughput analysis platforms, ISOLUTE SLE+ was demonstrated as a viable solution in the sample preparation workflow for vitamin D applications.

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47 drugs of abuse that can be extracted with Supported Liquid Extraction (SLE)
This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to UPLC-MS/MS analysis.

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Biotage ISOLUTE SLE+ applications
The ISOLUTE SLE+ applications e-book is designed to give an easy to use overview of the wide range of ISOLUTE SLE+ applications available.

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Biotage analytical sample preparation 2017
This catalog presents the extensive range of products for analytical sample preparation from Biotage.

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Application notes


Biotage ISOLUTE SLE+ applications
The ISOLUTE SLE+ applications e-book is designed to give an easy to use overview of the wide range of ISOLUTE SLE+ applications available.

Read more >>
PDF icon Download Application note, Converting a Liquid-Liquid Extraction method for Vitamin D to a 96-well plate Supported Liquid Extraction format.pdf (373.95 KB)
Converting a liquid-Lliquid Extraction method for vitamin D to a Supported Liquid Extraction plate format
Date of creation: 
30 December 2015
Library code: 
10566
PDF icon Download Application note, Extraction of 47 drugs of abuse from oral fluid by Biotage ISOLUTE SLE+ columns and plates.pdf (1.69 MB)
47 drugs of abuse that can be extracted with Supported Liquid Extraction (SLE)
Date of creation: 
13 January 2016
Library code: 
10582
PDF icon Download Application note, Extraction of cocaine and metabolites from whole blood using ISOLUTE SLE+ prior to GC-MS analysis.pdf (687.88 KB)
Extraction of cocaine and metabolites from whole blood using ISOLUTE SLE+ prior to GC-MS analysis
Date of creation: 
14 January 2016
Library code: 
10583
PDF icon Download Application note, Extraction of opiates from whole blood using SLE.pdf (698.56 KB)
Extraction of opiates from whole blood using ISOLUTE SLE+ prior to GC-MS analysis
Date of creation: 
14 January 2016
Library code: 
10585
PDF icon Download Application note, Extraction of THC, Hydroxy-THC and Carboxy-THC from whole blood by SLE.pdf (686.18 KB)
Extraction of THC, Hydroxy-THC and Carboxy-THC from whole blood using ISOLUTE SLE+ prior to GC-MS analysis
Date of creation: 
14 January 2016
Library code: 
10587
PDF icon Download Application note, Extraction of NBOMe designer drugs from oral fluid using ISOLUTE SLE+.pdf (1.39 MB)
Extraction of NBOMe designer drugs from oral fluid using ISOLUTE SLE+ prior to analysis by LDTD-MS/MS
Date of creation: 
14 January 2016
Library code: 
10588
PDF icon Download Application note, Extraction of amphetamines and analogues from hair using ISOLUTE SLE+ prior to GC-MS analysis.pdf (875.53 KB)
Extraction of amphetamines and analogues from hair using ISOLUTE SLE+ prior to GC/MS analysis
Date of creation: 
27 September 2017
Library code: 
11288
PDF icon Download Application note, Extraction of illicit drugs and pesticides from liver tissue using ISOLUTE SLE+ prior to GC-MS analysis.pdf (2.32 MB)
Extraction of illicit drugs and pesticides from liver tissue using ISOLUTE SLE+ prior to GC/MS analysis
Date of creation: 
27 September 2017
Library code: 
11289
PDF icon Download Application note, Acidic, basic and neutral drug screen of hydrolyzed urine using supported liquid extraction prior to LC-MS-MS analysis.pdf (906.41 KB)
Acidic, basic and neutral drug screen of hydrolyzed urine using supported liquid extraction prior to LC-MS/MS analysis
Date of creation: 
28 September 2017
Library code: 
11291
Literature

Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.

Readmore >>
PDF icon Download Technical note, Improve productivity and maximize analyte recovery with Biotage ISOLUTE SLE+ Supported Liquid Extraction plates and columns.pdf (344.44 KB)
Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns
Date of creation: 
30 December 2015
Library code: 
10563
PDF icon Download Data sheet, Biotage ISOLUTE SLE+ 48-Well Supported Liquid Extraction plate.pdf (1.09 MB)
Biotage ISOLUTE SLE+ 48-well Supported Liquid Extraction plate
Date of creation: 
30 December 2015
Library code: 
10565
PDF icon Download User guide, Biotage ISOLUTE SLE+ Supported Liquid Extraction.pdf (1.98 MB)
Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
Date of creation: 
30 December 2015
Library code: 
10568
PDF icon Download Poster, Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology.pdf (937.72 KB)
Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology
Date of creation: 
22 August 2017
Library code: 
11221
PDF icon Download Technical note, Improve productivity and maximize analyte recovery with Biotage ISOLUTE SLE+ Supported Liquid Extraction plates and columns.pdf (344.44 KB)
Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns
Date of creation: 
30 December 2015
Library code: 
10563
PDF icon Download Data sheet, Biotage ISOLUTE SLE+ 48-Well Supported Liquid Extraction plate.pdf (1.09 MB)
Biotage ISOLUTE SLE+ 48-well Supported Liquid Extraction plate
Date of creation: 
30 December 2015
Library code: 
10565
PDF icon Download Brochure, Biotage Extrahera Sample Preparation Automation System.pdf (2.67 MB)
Biotage Extrahera Sample Preparation Automation System
Date of creation: 
3 December 2015
Library code: 
10482
PDF icon Download Brochure, Biotage Extrahera sample preparation automation system.pdf (4.48 MB)
Biotage Extrahera sample preparation automation system
Date of creation: 
24 August 2017
Library code: 
11231
PDF icon Download Catalog, Biotage analytical sample preparation 2017.pdf (4.78 MB)
Biotage analytical sample preparation 2017
Date of creation: 
22 August 2017
Library code: 
11216