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Characterization of nanoparticles

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Overview

Characterization of nanoparticles by Field-Flow Fractionation (FFF)
During the past decades, FFF has been applied successfully for the separation, characterization and fractionation of a wide variety of large-size materials, including nanopartcles.

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Postnova EAF2000 electrical asymmetrical flow FFF system
The Postnova EAF2000 system is based on the AF2000 asymmetrical flow FFF module, with an additional Electrical FFF module, a special EAF4 channel and a software module. The EAF2000 is ideal for many applications in the domains of biopharm, environment and nanomaterials.

Postnova AF2000 MT mid temperature MultiFlow FFF system
The system can be configured for Asymmetric Flow Field-Flow Factionation (AF4), Hollow Fiber Flow Field-Flow Factionation (HF5) and Size Exclusion Chromatography (SEC).

Postnova CF2000 Centrifugal FFF system
The system can separate and distinguish relative size differences of 5% only and can be used to monitor absolute size differences down to 1 nm. These unique features make it the ideal choice for any nanoparticle research and QC tasks.

Postnova TF2000 Thermal FFF system
The system can separate simultaneously polymers and particles without the limitations associated with GPC/SEC. The separation takes place according to molecular weight AND chemical composition, thus making the TF2000 Series the ideal choice for difficult polymer separation.

Postnova 21-angle MALS detector - the ideal detector for ultra-high molar mass
The Postnova 21-angle MALS detector has been especially developed for coupling with FFF systems – to the opposite of previously available MALS detectors which were designed with chromatography (SEC/GPC) in mind, that is for smaller molar mass.

High sensitivity online Dynamic Light Scattering detector, special customized for use connected to FFF
Online DLS measurements using a special flow cell can be perfomed to obtain particle size (Rh) and molecular weight (Mw) distributions of particles and molecules separated by Postnova Asymmetric Flow FFF (AF4), Centrifugal FFF (CF3) and Thermal FFF (TF3) systems.

Application notes

Measurement of electrophoretic mobility of coated nanoparticles using EAF4
In this application note, the electrical asymmetrical flow FFF (EAF4) system was used to measure the electrophoretic mobility and zeta potential of 50 nm Ag nanoparticles (AgNPs) coated with three different coating materials, namely citrate, polyvinyl pyrrolidone (PVP) and tannic acid.

Separation of nanoplastics and determination of their surface charge by EAF4
Date of creation: 
28 November 2019
Library code: 
12742
Sizing of polydisperse silver nanoparticle suspensions - comparison of AF4 coupled to online DLS with batch mode DLS
Date of creation: 
28 November 2019
Library code: 
12740
Literature

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Postnova EAF2000 electrical asymmetrical flow FFF system, a universal particle/polymer/protein separator
Date of creation: 
3 September 2018
Library code: 
12204
Date of creation: 
24 July 2017
Library code: 
10163
Date of creation: 
29 September 2015
Library code: 
10159
Date of creation: 
1 October 2015
Library code: 
10164
The system can separate simultaneously polymers and particles without the limitations associated with GPC/SEC
Date of creation: 
1 October 2015
Library code: 
10165
Multi-Angle Light Scattering detector (MALS) model 3621
Date of creation: 
27 July 2017
Library code: 
10220
Dynamic Light Scattering detector model Zetasizer Nano ZS173
Date of creation: 
1 October 2015
Library code: 
10221