Evaluation of structural change and thermal stability of Bovine IgG

Evaluating the structure and stability of protein is extremely important for utilization in biopharmaceutical and such industries. CD spectra in far-ultraviolet region reflects to the secondary structure of protein. Therefore CD spectra measurement in far-ultraviolet region is widely used for evaluation of protein structure and it’s stability. As optical absorption derived from buffer solution in far-ultraviolet region is strong, it is necessary to contrive some preparative techniques so as to perform the measurement properly. In general, reducing buffer concentration and using a short path length cell are more effective to the measurement. PTC-510 water-cooled peltier cell holder has design side capability to fit with short path length cylindrical cell, which made it possible to measure CD spectrum of protein sample in buffer down to far-ultraviolet region. In this application note, CD spectra measurement of Bovine IgG / phosphate buffer solution was performed using Temperature Interval Scan Measurement Program Program and a short path length cylindrical cylindrical cell. Measurement temperature was set from 20ºC to 95ºC.