High-speed separation of steroid drug cortisone acetate utilizing X-LC

Cortisone acetate, a steroid, is administered to reduce tissue inflammation or to suppress the human immune system. The U.S. Pharmacopeia (USP)1) method requires that HPLC analysis of components of a cortisone acetate drug should have a resolution, R, between the analyte and internal standard peaks to be greater than 2.2 and the relative standard deviation for replicate injections to be not greater than 2.0%.

Jasco examined the utility of an X-PressPak C18S column (2.1 mm I.D. x 50 mm L.) packed with 2 um diameter packing material for the ultra high-speed separation of cortisone acetate. The results were examined to determine whether the performance of the column and chromatography separation meets the USP requirements.