CD spectrum and CD chromatogram measurement of warfarin using J-1500

HPLC is a more effective method for quantitative estimation of trace amounts of compounds and for mixture separation/purification. HPLC is also used for analysis of medicinal products and in food science. In these fields, samples often have optical activity allowing analysis of enantiomeric excess and the separation/purification of the enantiomers. Jasco has developed both dedicated CD detectors for HPLC as well as an HPLC flow cell for the J-1500/J-1700 CD Spectrometers. The J-1500/J-1700 provide high sensitivity, high resolution, and wide dynamic range, allowing high accuracy detection down to 200 nm.

In this report, the CD measurement of warfarin was carried out using the J-1500 in a static mode as well as coupled to an HPLC system for on-line CD detection of the HPLC eluent.