Norlab

Your partner in Northern Europe for innovative laboratory instruments

Automated fractionation of Extractable Petroleum Hydrocarbons (EPH) in soil using ISOLUTE EPH SPE columns on Biotage Extrahera

Resource type: 
Application note
Author(s): 
Biotage
Format: 
pdf
Date of creation: 
1 August 2017
Library code: 
11157

ISOLUTE EPH SPE columns and associated methodologies have been optimized to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions (C8–C40 aliphatics, C10–C22 aromatics).

The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimized. This allows an automation-compatible method of fractionation which overcomes the common problem of PAH breakthrough into the aliphatic fraction, in addition to reduced solvent volumes.

This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE EPH column in conjunction with the Biotage Extrahera automation system.

PDF icon Download Application note, Automated fractionation of Extractable Petroleum Hydrocarbons (EPH) in soil using ISOLUTE EPH SPE columns on Biotage Extrahera.pdf (2.49 MB)