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Extraction of phosphatidylethanol (PEth) species from whole blood using ISOLUTE SLE+ prior to HPLC-MS/MS analysis

Resource type: 
Application note
Author(s): 
Biotage
Format: 
pdf
Date of creation: 
1 August 2017
Library code: 
11158

Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE SLE+ supported liquid extraction prior to HPLC-MS/MS analysis.

ISOLUTE SLE+ supported liquid extraction fixed-well plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.

This simple sample preparation procedure produces clean extracts, good recoveries with low RSD, and LOQ from 20 ng mL-1. This method can be automated using Biotage Extrahera, see appendix for details.

PDF icon Download Application note, Extraction of phosphatidylethanol (PEth) species from whole blood using ISOLUTE SLE+ prior to HPLC-MS-MS analysis.pdf (2.64 MB)