Extraction of methylmalonic acid from serum using ISOLUTE SLE+ prior to LC-MS-MS analysis
Resource type:
Application note
Format:
pdf
Date of creation:
1 August 2017
Library code:
11161
Methylmalonic acid (MMA) in serum is measured to help diagnose a number of disorders, primarily vitamin B12 deficiency. This application note describes the extraction of methylmalonic acid from serum, prior to LC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized for the 200 uL capacity 96-well plate format is used. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs below 10%.
ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
