Extraction of amphetamines and analogues from hair using ISOLUTE SLE+ prior to GC/MS analysis

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. An effective and efficient ISOLUTE SLE+ protocol optimized for 1 mL capacity formats is detailed. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 72% with RSDs lower than 10% for all analytes. In addition, this note describes how to achieve quantitation of all analytes down to 0.2 ng/mg of hair. This complies with the LOQ values for laboratories involved in amphetamine testing, as suggested by the Society of Hair Testing (SoHT).