Acidic, basic and neutral drug screen of hydrolyzed urine using supported liquid extraction prior to LC-MS/MS analysis

ISOLUTE SLE+ Supported Liquid Extraction products offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.

This application note describes the extraction of acidic, basica and neutral drugs from urine for screening purposes using ISOLUTE SLE+ supported liquid extraction plates prior to LC-MS/MS analysis, ISOLUTE SLE+ is used to quickly screen a panel of drugs from a small amount of urine (100 uL). The limit of detection was determined for each analyte and was found to range from 5 ng/mL to 1.25 ng/mL. The application note describes this quick robust screening assay for the extraction of 32 different drugs from a hydrolyzed urine matrix.