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Extraction of barbiturates from oral fluid using ISOLUTE SLE+ after collection with the Oral-Eze collection device prior to GC/MS analysis

Resource type: 
Application note
Author(s): 
Biotage
Format: 
pdf
Date of creation: 
21 October 2017
Library code: 
11309

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bio-analytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of butalbarbital, butabarbital, amobarbital, pentobarbital, secobarbital, hexobarbital and phenobarbital from oral fluid matrix collected using the Oral-Eze collection devices, prior to GC/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized for the 400 uL sample capacity column format is described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 95% with RSDs lower than 9% for all analytes.

PDF icon Download Application note, Extraction of barbiturates from oral fluid using ISOLUTE SLE+ after collection with the Oral-Eze collection device prior to GC-MS analysis.pdf (1.19 MB)