Extraction of THC, THCA and carboxy-THC from oral fluid by ISOLUTE SLE+ after collection with the Quantisal collection device prior to GC/MS analysis

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of THC, THCA and carboxy-THC from oral fluid matrix collected using the Quantisall (immunalysis) device, prior to GC/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized for 400 uL and 1 mL sample capacity formats is described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 75% with RSDs lower than 10% for all analytes.