Extraction of barbiturates from oral fluid using ISOLUTE SLE+ after collection with the Quantisal collection device prior to GC/MS analysis

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of butalbarbital, butabarbital, amobarbital, pentobarbital, secobarbital, hexobarbital and phenobarbital from oral fluid matrix collected using Quantisal collection devices (immunalysis), prior to GC/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized for 400 uL and 1 mL sample capacity formats is described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 88% with RSDs lower than 6% for all analytes.