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Extraction of barbiturates from oral fluid using ISOLUTE SLE+ after collection with the Intercept oral fluid drug test kit prior to GC/MS analysis

Resource type: 
Application note
Author(s): 
Biotage
Format: 
pdf
Date of creation: 
21 October 2017
Library code: 
11312

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bio-analytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of butalbarbital, butabarbital, amobarbital, pentobarbital, secobarbital, hexobarbital and phenobarbital from oral fluid matrix collected using the Intercept oral fluid drug test kit (Orasure Technologies), prior to GC/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized for 400 uL and 1 mL sample capacity formats is described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 7% for all analytes.

PDF icon Download Application note, Extraction of barbiturates from oral fluid using ISOLUTE SLE+ after collection with the Intercept oral fluid drug test kit prior to GC-MS analysis.pdf (1.63 MB)