Extraction of 45 multi class drugs of abuse from urine (non-hydrolyzed) using ISOLUTE SLE+ prior to LC-MS/MS analysis

ISOLUTE SLE+ products provide clean, rapid, robust, efficient, high throughput and automatable extraction solutions for a wide range of analytes.

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of various drugs of abuse from non-hydrolyzed urine prior to LC-MS/MS analysis. The method described achieves high reproducible recoveries for a wide range of drugs of abuse and metabolites from non-hydrolyzed urine. Urine was extracted using the ISOLUTE SLE+ 200 fixed well plate and 1 mL column formats using 100 and 500 uL sample volumes, respectively. Sub ng/mL levels were achieved for all analytes using 100 uL of urine on the fixed well plate format.