Extraction of cocaine and metabolites from hydrolyzed urine using ISOLUTE SLE+ prior to GC-MS analysis

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of cocaine and a range of cocaine metabolites from hydrolyzed human urine prior to GC-MS. An effective and efficient ISOLUTE SLE+ protocols optimized for sample loading volumes of either 400 uL or 1 mL are described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <7% for all analytes.