Extraction of cocaine and metabolites from urine using ISOLUTE SLE+ prior to LC-MS/MS analysis

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation time.

This application note describes the extraction of cocaine and a full range of cocaine metabolites from human urine prior to analysis and quantitation by LC-MS/MS. Effective and efficient ISOLUTE SLE+ protocols optimized for sample (urine) volumes of either 200 uL or 1 mL are described. Procedures for high throughput (96-well plate) and column format are included.