Extraction of testosterone and other steroid hormones from human plasma using ISOLUTE SLE+ 96-well plates

ISOLUTE SLE+ supported liquid extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation time.

The method in this application describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) to extract a range of steroid hormones including testosterone and progesterone from human plasma. This simplified and efficient extraction method has significant analyte recoveries ranging from 90-107% with LOQs as low as 500 pg/mL.