Extraction of tricyclic anti-depressants from plasma using ISOLUTE SLE+ supported liquid extraction plates

The supported liquid extraction (SLE) process is analogous to traditional liquid-liquid extraction (LLE) and utilizes the same water immiscible solvent systems for analyte extraction.  Instead of shaking the two immiscible phases together, in SLE, the aqueous sample is immobilised on an inert support and the organic phase flows through the support, eliminating problems such as emulsion formation and low analyte recoveries.

This application note describes the development of an automated procedure for high throughput supported liquid extraction of three tricyclic antidepressant drugs from human plasma, using the ISOLUTE SLE+ supported liquid extraction plate. Analyte recovery, along with the speed and efficiency are compared to traditional LLE.