Characterisation of monoclonal antibody aggregates using AF4-UV/Vis
Resource type:
Application note
Format:
pdf
Date of creation:
20 August 2018
Library code:
12164
Although asymmetric flow FFF offers similar information as SEC, the lack of packing material leads to significant advantages for AF4, specifically low shear conditions so that aggregates are not degraded. Versus ultracentrifugation, the AF4 system is easier to operate, easily suited for autosampler use and/or for collection of fractionation. Additionally, the AF4 separation is based on the diffusion coefficient of the various species. Thus, direct measurement of diffusion coefficient and hydrodynamic radius information is also possible.
