Centrifugal field-flow fractionation hyphenated with dynamic light scattering for size characterization of nanoparticle systems for biopharmaceutical applications

The hyphenation of centrifugal FFF with dynamic light scattering has proven to be an ideal tool for the investigation of particle sizes even in complex matrices such as e.g., cell medium. CF3-DLS thereby for the first time not only enabled the detection, but also the evaluation of the particle integrity as well as the monitoring of particle size alterations of PLGA NPs directly in cell medium over time. Thus, CF3-DLS gives valuable information on the potential of biocompatible PLGA NPs towards their application in drug delivery systems (DDS). Therefore, this technology can provide detailed insights into NP behavior under physiological conditions, where other particle sizing technologies such as batch-DLS usually fail.