Automated NGS library prep: ligation efficiency of NxSeq sample preparation using the Gilson Pipetmax 268
Resource type:
Application note
Format:
pdf
Date of creation:
29 May 2020
Library code:
12998
The Gilson Pipetmax is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility and consistency among all samples processed. Unlike traditional automation platforms, Pipetmax comes in a benchtop size that easily fits into any lab.
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAM-labeled adaptors using the NxSeq DNA sample preparation kit, comparing the manual method with an automated library prep protocol on the Pipetmax. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.
