Automating the Promega GoTaq PCR core system II reaction using the Gilson Pipetmax 268

The Gilson Pipetmax is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility and consistency among all samples processed. Unlike traditional automation platforms, Pipetmax comes in a benchtop size that easily fits into any lab.

In this application, the compact Pipetmax 268, a reproducible set-up, run and walk-away sample prep assistant, was used to prepare polymerase chain reactions (PCRs) with the GoTaq PCR sore system II, and to compare its effectiveness versus a manually pipetted procedure. Positive and negative control reactions were set up and amplified using the manufacturer’s recommended guidelines, and analyzed by agarose gel electrophoresis. As shown, the Pipetmax successfully generated the expected 323 bp product in the positive control reactions, and showed no contamination or carryover in the no template control (NTC).