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Optimization of qPCR master mix dispensing with Pipetmax by Gilson

Resource type: 
Application note
Author(s): 
Gilson
Format: 
pdf
Date of creation: 
31 May 2020
Library code: 
13004

The Gilson Pipetmax is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility and consistency among all samples processed. Unlike traditional automation platforms, Pipetmax comes in a benchtop size that easily fits into any lab.

In this application note, Gilson used the Pipetmax automated liquid handler to test whether the number of times a tip was pre-wet before transferring master mix affected qPCR performance. Increasing the number of pre-wet steps resulted in improved qPCR sample replicate uniformity, demonstrating the importance of proper pipetting technique according to the characteristics of the liquid. The flexibility and consistency of the Pipetmax increases data reliability, enabling verifiable science through easily integrated automation.