Lipidomics decoded: targeted assignment of polar lipids using the SICRIT LC-module

This application note shows through a simple LC-HR-MS/MS study how using the SICRIT ionization technique, you can conduct successful analysis of a wide range of polar lipids. Additionally, you can obtain a usable MS2 spectra that can be used for either targeted or untargeted analysis. This is shown using the Assign functionality in LabSolution, which uses an untargeted online search approach to match the produced MS2 to the compound, when provided minimal information (precursor mass or proposed formula). This allows us to fully streamline both targeted and untargeted approaches for lipidomics or metabolomics analysis, similar to standard ionization techniques used in most pipelines.