Biotage ISOLUTE PPT+ protein precipitation plates

Protein precipitation by filtration

Protein precipitation is a routinely used sample preparation technique for removal of proteins from biological
fluid samples prior to analysis. Protein precipitation in the 96-well format, using filtration for protein removal,
is a high throughput alternative to the traditional centrifugation based technique.

The design of most filterplates requires the plasma sample to be dispensed into the well before the precipitating
solvent can be added. This approach can lead to cloudy extracts caused by incomplete precipitation. Successful
protein removal can depend on difficult to automate factors such as the speed of solvent delivery, vortex mixing
or inversion of the plate. Methods can be non-robust and well blockage is common.

Sample preparation using ISOLUTE PPT+ Protein Precipitation Plates is fast and simple. The optimized
filtration system in ISOLUTE PPT+ plates provides an easy to automate solution for efficient protein
removal. The procedure is streamlined,with no awkward off-line steps.