GL Sciences MonoCap capillary columns

MonoCap HighResolution 2000 Series

Optimized for identification of peptides/proteins for proteome research

MonoCap HighResolution 2000 is a 2 meter length monolithic silica capillary column which is designed for identifying extremely high number of peptides/proteins for proteome research via LC-MS/MS.

GL Sciences’ MonoCap capillary columns, created synthetically via sol-gel method, and an octadecyl silane chemically bonded, has a very uniform three dimensional structure that shows excellent reproducibility from batch-to-batch.

The solid structure of GL Sciences’ monolithic silica eliminates the need for frits or filters at the ends of the column, thereby reducing dead volume that might otherwise lead to band broadening or sample recovery.

The high porosity of our monolithic silica allows high flow rates to be used without loss of resolutionor creation of high operating pressure. An optimized balance of through-pores and meso-pores provides the critically important combination of efficiency, separation speed, large volume sample-loading, and small volume sample-recovery.

MonoCap HighResolution provide extremely high efficiency, delivering over 200,000 plates for a 2,000 mm length column. The MonoCap HighResolution Ultra type deliver over 300,000 plates.

Discover new peptides/proteins with MonoCap C18 HighResolution 2000

MonoCap C18 HighResolution 2000 mm length column identifies simply more peptides/proteins compared to those traditional particle packed capillary HPLC columns.

MonoCap HILIC-UP for identifying highly hydrophilic, hydrophobic peptides/proteins

MonoCap HILIC-UP is an important addition to the MonoCap C18 HighResolution 2000 column series. MonoCap HILIC-UP can retain highly hydrophilic peptides/proteins which may lead to discovering new peptides/proteins where a C18 phase couldn’t identify.

In HILIC, the higher the organic concentration, the greater the retention of more polar analytes. One of the biggest benefit of HILIC mode is, a high organic solvent concentration of the mobile phase will lead to a high sensitivity LCMS/MS analysis.

Other MonoCap Series columns

MonoCap C18 Fast-flow

Workable at a broad range of linear velocity from 0.5 to 5 mm/s without sacrificing efficiency and separation at high speed. The number of theoretical plates produced by MonoCap C18 Fast-flow is nearly equivalent to a totally porous particle type capillary column packed with a 5 μm packing material. 

MonoCap C18 Nano-flow

MonoCap C18 Nano-flow produces higher number of theoretical plates compared to a totally porous particle type capillary column packed with a 3 μm packing material. It can be operated at a wide range of flow rate with low back pressure and achieve very high sensitive results in Nano-LC-ESI/MS applications. 

MonoCap C18 WideBore

The MonoCap C18 Fast-flow is also available in 0.5 mm I.D. size, which can be used at a wide range of fl ow rate from 6 to 100 μL/min without sacrificing efficiency. The number of theoretical plates produced by MonoCap C18 WideBore is nearly equivalent to a totally porous particle type capillary column packed with a 5 μm packing material. 

MonoCap C18 Trap Column

MonoCap Trap columns have a relatively big throughpore and workable at a high flow rate such as 10 μL/min. This benefit makes MonoCap Trap columns to be appropriate for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection. End-fittings are 1/16” (10-32 UNF). 1/32” end-fittings are also available upon request.

MonoCap SCX

MonoCap SCX is bonded with benzene sulfonic acid groups (strong cation exchange) and appropriate for 2D LC applications for the separation of biomolecules such as peptides and proteins.