Extraction of 25-hydroxy vitamin D from serum using ISOLUTE PLD+ prior to LC-MS/MS analysis
Resource type:
Application note
Format:
pdf
Date of creation:
20 August 2017
Library code:
11214
ISOLUTE PLD+ protein and phospholipid removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation.
This application note describes a simple, effective ISOLUTE PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
