Extraction of estrone and estradiol from human serum using ISOLUTE SLE+ prior to HPLC-MS/MS

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

Estrone (E1) and estradiol (E2) are female steroid hormones typically monitored in peri- and postmenopausal human serum samples to assess a number of conditions.

This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used. High, reproducible analyte recovery and low detection limits are achieved.