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Extraction of 11-nor-9-carboxy-tetrahydrocannabinol from hydrolyzed urine by ISOLUTE SLE+ prior to GC/MS analysis

Resource type: 
Application note
Author(s): 
Biotage
Format: 
pdf
Date of creation: 
17 October 2017
Library code: 
11303

ISOLUTE Supported Liquid Extraction (SLE+) plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation. 11-nor-9-carboxy-tetrahydrocannabinol (carboxy-THC; THC-COOH) is the primary metabolite of THC. In urine, 80% of THC-COOH is present in the form of its glucuronide metabolite. Therefore, to effectively quantitate the THC-COOH, urine is hydrolyzed before extraction.

This application note describes optimized extraction of urine samples prepared by either enzymatic or base hydrolysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 87% with RSDs lower than 10% for all analytes, and is optimized for use with 1 mL sample capacity ISOLUTE SLE+ columns.

PDF icon Download Application note, Extraction of 11-nor-9-carboxy-tetrahydrocannabinol from hydrolyzed urine by ISOLUTE SLE+ prior to GC-MS analysis.pdf (798.85 KB)