Analysis of THC and an extended metabolite suite from oral fluid using ISOLUTE SLE+ Supported Liquid Extraction columns prior to LC-MS/MS
Resource type:
Application note
Format:
pdf
Date of creation:
23 October 2017
Library code:
11315
ISOLUTE SLE+ products provide clean, rapid, robust, efficient, high throughput and automatable extraction solutions for a wide range of analytes.
The method described in this application note achieves high recoveries of THC and an extended suite of common metabolites in oral fluid from Quantisal (immunalysis) oral fluid collection devices. Effective and efficient ISOLUTE SLE+ protocols optimized for sample loading volumes of either 300 uL or 800 uL are described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 64% with RSDs of <10% for all analytes.
