Extraction of a comprehensive steroid panel from horse hair using ISOLUTE SLE+ prior to LC-MS-MS analysis

Biotage ISOLUTE SLE+ supported liquid extraction plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of a panel of 18 steroid hormones from horse hair using ISOLUTE SLE+ supported liquid extraction plates prior to LC-MS-MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 5% for most analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 0.5-500 pg/mg of hair.