Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
Resource type:
User guide
Format:
pdf
Date of creation:
30 December 2015
Library code:
10568
This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.
ISOLUTE SLE+ plates and columns contain a modified form of diatomaceous earth, and are used for the extraction of a diverse range of analytes from aqueous samples such as biological fluids, using a simple load-wait-elute procedure. Various formats, both well plates and columns are available for the extraction of aqueous sample volumes ranging from 10 μL to 10 mL.
Contents of the ISOLUTE SLE+ guide
- Sample preparation using Supported Liquid Extraction
- Extraction mechanism
- Extraction cleanliness
- Phospholipid and protein removal using ISOLUTE SLE+
- Productivity in automation
- Processing ISOLUTE SLE+ products
- Processing conditions
- Volume guidelines
- Internal standards
- Scalability
- Method development strategies
- Sample pre-treatment
- Method development for neutral (non-ionizable) compounds
- Method development for basic compounds
- Method development for acidic compounds
- Streamlined method development
- Extending the range of extractable analytes
- Hints, tips and troubleshooting
- Applications listing
- Biotage sample processing accessories
- Biotage evaporation products
