High-throughput CD spectral measurement for the evaluation of pH dependency of biomedicines

Biomedicine is a medicinal response to a physical or biological issue with the human body. Biomedicines use active ingredients derived from proteins and the research and development behind these methods is increasing daily. However, these biomedicines are more sensitive to environmental changes, such as a change in temperature, pH, and salt concentration, than traditional pharmaceuticals. This environmental sensitivity may be a potential cause of biomedicines’ deactivation during production and storage processes.

Circular dichroism (CD) measurements can provide information regarding changes in protein structure in small quantities of sample. Since protein structure and activity are closely related, CD measurements are now widely accepted in the quality control of protein, which includes biomedicines.

To meet the demand for increased sample throughput in the modern pharmaceutical laboratory, Jasco has developed a fully automated high-throughput CD spectral measurement system. This system is composed of a J-1500 CD spectrometer with an autosampler unit. The high-throughput CD system enables the automation of sample pretreatment, injection and washing.

In this application note, Jasco will demonstrate the use of the ASU-800 automated system to evaluate the pH dependency on the structure of human serum albumin (HSA).