Mapping analysis of secondary structure of protein using microscope FTIR

Jasco developed new programs for secondary structure analysis of proteins from IR spectra (which is called IR-SSE). Presently, for the secondary structure estimation of proteins, the structural analyses of crystallized proteins using X-ray and the methods utilizing CD (Circular Dichroism) for proteins in aqueous solution, are widely used. However, it has been extremely difficult to estimate secondary structure of multicomponent proteins such as biological tissue. On the other hand, the method using FTIR enables secondary structure analyses of proteins contained in biological tissue. Moreover, using microscope FTIR enables mapping analysis of nonuniform samples.

The distribution maps of secondary structures of proteins, based on the data of the mapping measurement using microscope FTIR, are shown in this application data.