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Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.

Biotage ISOLUTE SLE+ applications
The ISOLUTE SLE+ applications e-book is designed to give an easy to use overview of the wide range of ISOLUTE SLE+ applications available.

Converting a liquid-liquid extraction method for vitamin D to a Supported Liquid Extraction plate format
The objective of this study was to determine feasibility of transferring a validated Liquid-Liquid Extraction (LLE) method to a Supported Liquid Extraction (SLE) method using Biotage ISOLUTE SLE+ 96-well plates (400 uL sample capacity). The laboratory supporting this study processes ~550 samples per month supporting vitamin D analysis in plasma and serum (mixed samples / not separated). During this evaluation, it was determined that sample processing time was reduced by ~50% for a de-identified patient set (n=30, split and processed by both methods). Towards high throughput analysis platforms, ISOLUTE SLE+ was demonstrated as a viable solution in the sample preparation workflow for vitamin D applications.

Biotage ISOLUTE SLE+ 48-well Supported Liquid Extraction plate
ISOLUTE SLE+ is available in high volume 48-well plate format, with capacity for up to 1 mL of aqueous sample, such as urine or plasma, per well. Ideal for high throughput applications where extra sample capacity is required, ISOLUTE SLE+ 1 mL capacity plates can help you to achieve higher sensitivity and lower detection limits.

Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns
ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional Liquid Liquid Extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion formation and reduces sample preparation time by half.

Biotage ISOLUTE QuEChERS extraction and clean up tubes
Biotage QuEChERS products include extraction tubes for the initial extraction and partitioning of analytes from homogenized food samples, and clean up tubes for the dispersive solid phase extraction. All Biotage QuEChERS products come packed in a rack for ease of storage and use on the bench. Additionally, solvent resistant paper labels aid hassle free sample indentification. Biotage supplies pre-weighed extraction and clean up tubes that conform with AOAC1,2 and the EU3 published methods, including those for waxed and highly pigmented fruit and vegetable samples.

Biotage ISOLUTE Myco SPE columns
One product with multiple applications, simplifying and streamlining mycotoxin analysis procedures.

Biotage ISOLUTE ENV+ SPE columns and plates
ISOLUTE ENV+ is a highly retentive non-polar SPE phase, for the extraction of polar analytes that are not retained by C18 or C8 sorbents. Originally developed for environmental analysis, ISOLUTE ENV+ is also the SPE product of choice for a variety of agrochemical and food applications.

Sample preparation using ISOLUTE PPT+ protein precipitation plates
This chemistry data sheet describes the use of ISOLUTE® PPT+ Protein Precipitation Plates for the high
throughput sample preparation of biological fluids (plasma, serum or blood).

Biotage ISOLUTE PPT+ protein precipitation plates
Sample preparation using ISOLUTE PPT+ Protein Precipitation Plates is fast and simple. The optimized filtration system in ISOLUTE PPT+ plates provides an easy to automate solution for efficient protein removal. The procedure is streamlined,with no awkward off-line steps.

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