Streamlined sample preparation of a drugs of abuse panel in human nails using ISOLUTE SLE+ prior to UHPLC-MS-MS analysis
Resource type:
Application note
Format:
pdf
Date of creation:
20 August 2019
Library code:
12647
Biotage ISOLUTE SLE+ supported liquid extraction plates and columns are designed to provide stress free extraction of analytes from biological fluids, with high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop and automation is simple.
This application note contains procedures optimized for both individual column format and 96-well plate format for higher throughput applications. The methodology delivers clean extracts and analyte recoveries mostly greater than 80% with RSDs lower than 10% for all analytes and LLOQ from 1 pg/mg.
