Extraction of a drugs of abuse panel from oral fluid using ISOLUTE SLE+ after collection with the Intercept oral fluid drug test kit prior to UPLC-MS-MS analysis

This application note describes the extraction of 47 drugs of abuse from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized for the 1 mL capacity column format is described.

ISOLUTE SLE+ Supported Liquid Extraction columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.