Determination of 1,4-dioxane using Horizon 5000 automated SPE system

The Biotage Horizon 5000 is a three-position automated disk extraction system that conditions the disk, loads the sample (20 mL to 2 L) and elutes the analytes all without user intervention.

This application note proves that EPA method 522 can be successfully implemented in a laboratory using the Biotage Horizon 5000 automated solid phase extraction system. Four LFB samples were analyzed for precision and accuracy, yielding an average recovery value of 85.25% with an RSD of 2.93%. Both values meet the acceptance criteria of the method. The batch to batch quality control requirements set by the EPA method are easily met using this extraction method. A blind performance test sample validated the accuracy of results obtained for drinking water. The automation of this method provides less analyst intervention which reduces any possible outside contamination. The 10 mL final extract volume eliminates any losses due to evaporation while the larger sorbent bed has allows for faster flow rate with better performance. All of these factors lead to an increase in productivity while easily meeting all of the quality control requirements for EPA method 522.