Measurement of protein film samples in vacuum ultraviolet region by using J-1500 CD spectrometer
CD spectra reflecting the secondary structure of protein are usually observed from UV region below 260 nm to the vacuum ultraviolet region. It has been reported that by CD instrument utilizing synchrotron radiation the protein film sample was successfully measured down to around 140 nm. CD analysis by using synchrotron radiation is a very effective method to measure CD in vacuum ultraviolet region, however, it is not easy since it requires a very special facility. JASCO Model J-1500 enables to achieve high S/N CD measurement even in vacuum ultraviolet region. This note shows the analysis example of protein film sample in vacuum ultraviolet region by using J-1500 CD spectrometer.