Higher-order structure analysis of high concentration monoclonal antibody by CD and IR spectroscopy
Jasco estimated the secondary structure contents of rabbit serum IgG at concentrations of 5.8 to 23.1 mg/mL from its far-UV CD and IR spectrum. For CD secondary structure analysis (SSE), they used the J-1500 CD spectrometer which can measure CD and absorption simultaneously, and the BeStSel algorithm which shows high accuracy of β-sheet estimation. For IR SSE, they used their latest FTIR spectrometer, FT/IR-4X and their improved IR SSE software, IR SSE-4000.