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A simple setup to use semi-preparative HPLC columns on Biotage Selekt
The Biotage Selekt can be run at a maximum flowrate of 300 mL/min or a maximum pressure of 30 bar. These high flowrates and pressures enable a user to perform chromatography using not only dry-packed, single-use plastic flash columns containing small (≥20 um) spherical silica particles, but also semi-preparative, slurry-packed HPLC columns for multiple use with smaller (≤20 um) spherical silica particles.

TF3 with MALS and viscometer – comprehensive two-dimensional polymer separation with advanced detection
Thermal field-flow fractionation (TF3) is a powerful technique for the separation of large or complex polymer samples. When used in conjunction with advanced detection systems such as multi angle light scattering (MALS) and viscosity, it is an indispensable tool to gain deeper insight into the nature of these complex polymer samples.

Separation of nanoplastics and determination of their surface charge by EAF4
In this application note, Postnova presents data on separation of polystyrene nanoplastics, and demonstrate how electrical asymmetrical flow FFF (EAF4) can be used for simultaneous size separation and particle surface charge measurement.

Quantification of adeno-associated virus aggregation using asymmetrical flow field-flow fractionation
In this application note, Postnova presents data on separation of AAVs and their aggregates using asymmetrical flow field-flow fractionation (AF4) and measurement of their radius of gyration (Rg or root mean square radius, R.M.S.) by multi angle light scattering (MALS).

Sizing of polydisperse silver nanoparticle suspensions - comparison of AF4 coupled to online DLS with batch mode DLS
This application note presents a comparison of DLS in batch mode with asymmetrical flow field-flow fractionation (AF4) coupled with DLS (AF4-DLS) for the measurement of a mixture of silver nanoparticles (AgNP) of different sizes (20 nm, 40 nm, 60 nm).

Measurement of cancer biomarker enrichment in exosomes: separation using both AF4 and CF3
In this study the goal was to characterize the MCF-7 tumor exosome sample by size using asymmetrical flow field-flow fractionation (AF4) coupled to multi angle light scattering (MALS), and by mass using centrifugal field-flow fractionation (CF3).

Measurement of electrophoretic mobility of coated nanoparticles using EAF4
In this application note, the electrical asymmetrical flow FFF (EAF4) system was used to measure the electrophoretic mobility and zeta potential of 50 nm Ag nanoparticles (AgNPs) coated with three different coating materials, namely citrate, polyvinyl pyrrolidone (PVP) and tannic acid.

Using a semi-preparative reversed phase HPLC column on Biotage Selekt
In this application note, Biotage shows the separation of a four-component mixture using a semi-preparative, slurry-packed reversed phase HPLC column with 15 um spherical silica particles and a Biotage Selekt instrument.

Using a semi-preparative normal phase HPLC column on Biotage Selekt
In this note, Biotage shows the separation of a three-component mixture using a semi-preparative slurry-packed HPLC column with 15 um spherical silica particles and a Biotage Selekt instrument.

Evaluation of matrix component removal using novel flow-through scavenging plates or columns for drugs of abuse testing in urine
This poster evaluates the extraction of a range of drugs of abuse from hydrolyzed and non-hydrolyzed urine using a novel flow through scavenging product, ISOLUTE HYDRO DME+.

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