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Recombinant antibodies application using AF4 - UV/Vis
Asymmetric Flow FFF (AF4) is the premier technique for the characterization of recombinant antibodies. The main reason for this is, that AF4 can characterize not only the antibody species there selves, but also their fragments and aggregates. Furthermore, even small differences in the conformation and the general heterogeneity of samples can be determined.

PlasmidDNA and DNA complex application using Flow FFF - UV/Vis
In this application, Flow FFF was used to isolate and subsequently characterize lipid–DNA complexes.

Liposomes application using AF4 - SLS/DLS
Using AF4, high resolution separation of nanoparticles, even a few nanometers can be distinguished. AF4 allows for a very fast, gentle and nearly interaction free separation without a stationary phase, and the separation of complex matrices can be accomplished without sample preparation or filtering.

Characterization of virus and aggregates using AF4-MALS
This application note shows how AF4 can be used for the fast (ca. 20 min) and high resolution characterization of viruses and their aggregates. This makes the technology an important standard tool in modern pharmaceutical/biotechnology sciences.

Characterisation of monoclonal antibody aggregates using AF4-UV/Vis
Although asymmetric flow FFF offers similar information as SEC, the lack of packing material leads to significant advantages for AF4, specifically low shear conditions so that aggregates are not degraded