Supported liquid extraction of vitamin D metabolites: 25-hydroxy and 1α, 25-dihydroxy vitamin D2/D3 using PTAD derivatization prior to LC-MS/MS analysis
In this poster, high analyte recoveries and low ion suppression are demonstrated, allowing limits of quantitation at low pg/mL levels for the di-OH metabolites and < 1 ng/mL levels for the OH metabolites.