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Extraction of low level testosterone and androstenedione from human serum samples using ISOLUTE SLE+
The method in this application has been optimized to extract testosterone and androstenedione from female clinical serum samples. It has achieved very low limits of quantitation for testosterone and androstenedione 0.4 nmol/L and 0.9 nmol/L respectively. The recoveries for both analytes were greater than 90%.

Extraction of a range of opiates and metabolites from human urine using ISOLUTE SLE+ columns prior to GC-MS analysis
An effective and efficient supported liquid extraction protocol for the clean-up and concentration of a range of forensically significant opiates and their metabolites is demonstrated in this application.

Extraction of cocaine and metabolites from urine using ISOLUTE SLE+ prior to LC-MS/MS analysis
Effective and efficient ISOLUTE SLE+ protocols optimized for sample (urine) volumes of either 200 uL or 1 mL are described in this application note. Procedures for high throughput (96-well plate) and column format are included.

Extraction of a range of amphetamines and metabolites from human urine using ISOLUTE SLE+ columns prior to GC-MS analysis
The methodology in this application has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of a range of forensically significant amphetamines followed by derivatization to optimize for GC-MS analysis.

Extraction of 45 multi class drugs of abuse from hydrolyzed urine using ISOLUTE SLE+ prior to LC-MS/MS analysis
The method described in this application note achieves high reproducible recoveries for a wide range of drugs of abuse and metabolites from hydrolyzed urine.